rabbit anti-sox9 Search Results


sox9 polyclonal antibody  (Bioss)
94
Bioss sox9 polyclonal antibody
Sox9 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sox9 polyclonal antibody/product/Bioss
Average 94 stars, based on 1 article reviews
sox9 polyclonal antibody - by Bioz Stars, 2026-02
94/100 stars
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sox9 antibody  (Bio-Techne corporation)
93
Bio-Techne corporation sox9 antibody
Sox9 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sox9 antibody/product/Bio-Techne corporation
Average 93 stars, based on 1 article reviews
sox9 antibody - by Bioz Stars, 2026-02
93/100 stars
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rabbit anti-sox9  (Merck & Co)
90
Merck & Co rabbit anti-sox9
Rabbit Anti Sox9, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-sox9/product/Merck & Co
Average 90 stars, based on 1 article reviews
rabbit anti-sox9 - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti-sox9  (Cell Marque)
90
Cell Marque rabbit anti-sox9
YAP inhibition reduces <t>Sox9</t> + progenitors in vitro. a Experimental design for b – e . b – d Flow cytometry of SOX9 expression in the control and verteporfin-treated differentiating progenitors and quantification of the proportion of SOX9 + cells from b and c at the end of stage 6 differentiation (stage 6, day 14). mCFP is an unstained control. Data represent mean ± SEM, ** p < 0.01 ( n = 3 biologically independent samples per group). e Proportion of Ki67 + in control or verteporfin-treated differentiating progenitors as assayed by flow cytometry at the end of stage 6 differentiation. Data represent mean ± SEM, *** p < 0.001 ( n = 3 biologically independent samples per group). f – h Flow cytometry analysis of SOX9 in non-targeting control and YAP shRNA-expressing pancreatic progenitors and quantification of SOX9 + cell proportion ( h ) at the end of stage 6 differentiation. Data represent mean ± SEM, *** p < 0.001 ( n = 3 biologically independent samples per group). i Experimental design for j – n . j , k in vivo GSIS of control and verteporfin-derived SC-β cell grafts as assayed by serum human insulin before and 30 min after a glucose injection 8 weeks post transplantation. l , m Immunofluorescence micrographs of grafts 12 weeks post transplantation of control and verteporfin-derived SC-β cell grafts stained for C-peptide and SOX9. Representative images and cropped blots (bottom panels) are shown. Asterisk (*) denotes nonspecific staining. Scale bar: 50 µm. n Quantification of SOX9 + of DAPI + cells within grafts 12 weeks after transplantation. Data represent mean (center line) ± min to max (whiskers) and lower and upper quartiles (bounds of box), *** p < 0.001, two-sided student’s t test ( n = 4 animals per group, values correspond to the average proportion of SOX9 + cells from four histological sections per animal)
Rabbit Anti Sox9, supplied by Cell Marque, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-sox9/product/Cell Marque
Average 90 stars, based on 1 article reviews
rabbit anti-sox9 - by Bioz Stars, 2026-02
90/100 stars
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sox9 primary antibodies  (Chemicom Inc)
90
Chemicom Inc sox9 primary antibodies
YAP inhibition reduces <t>Sox9</t> + progenitors in vitro. a Experimental design for b – e . b – d Flow cytometry of SOX9 expression in the control and verteporfin-treated differentiating progenitors and quantification of the proportion of SOX9 + cells from b and c at the end of stage 6 differentiation (stage 6, day 14). mCFP is an unstained control. Data represent mean ± SEM, ** p < 0.01 ( n = 3 biologically independent samples per group). e Proportion of Ki67 + in control or verteporfin-treated differentiating progenitors as assayed by flow cytometry at the end of stage 6 differentiation. Data represent mean ± SEM, *** p < 0.001 ( n = 3 biologically independent samples per group). f – h Flow cytometry analysis of SOX9 in non-targeting control and YAP shRNA-expressing pancreatic progenitors and quantification of SOX9 + cell proportion ( h ) at the end of stage 6 differentiation. Data represent mean ± SEM, *** p < 0.001 ( n = 3 biologically independent samples per group). i Experimental design for j – n . j , k in vivo GSIS of control and verteporfin-derived SC-β cell grafts as assayed by serum human insulin before and 30 min after a glucose injection 8 weeks post transplantation. l , m Immunofluorescence micrographs of grafts 12 weeks post transplantation of control and verteporfin-derived SC-β cell grafts stained for C-peptide and SOX9. Representative images and cropped blots (bottom panels) are shown. Asterisk (*) denotes nonspecific staining. Scale bar: 50 µm. n Quantification of SOX9 + of DAPI + cells within grafts 12 weeks after transplantation. Data represent mean (center line) ± min to max (whiskers) and lower and upper quartiles (bounds of box), *** p < 0.001, two-sided student’s t test ( n = 4 animals per group, values correspond to the average proportion of SOX9 + cells from four histological sections per animal)
Sox9 Primary Antibodies, supplied by Chemicom Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sox9 primary antibodies/product/Chemicom Inc
Average 90 stars, based on 1 article reviews
sox9 primary antibodies - by Bioz Stars, 2026-02
90/100 stars
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sox9 antibody  (Cosmo Bio USA)
90
Cosmo Bio USA sox9 antibody
Antibodies
Sox9 Antibody, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sox9 antibody/product/Cosmo Bio USA
Average 90 stars, based on 1 article reviews
sox9 antibody - by Bioz Stars, 2026-02
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anti-sox9 antibody  (Advisains)
99
Advisains anti-sox9 antibody
Antibodies
Anti Sox9 Antibody, supplied by Advisains, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-sox9 antibody/product/Advisains
Average 99 stars, based on 1 article reviews
anti-sox9 antibody - by Bioz Stars, 2026-02
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Image Search Results


YAP inhibition reduces Sox9 + progenitors in vitro. a Experimental design for b – e . b – d Flow cytometry of SOX9 expression in the control and verteporfin-treated differentiating progenitors and quantification of the proportion of SOX9 + cells from b and c at the end of stage 6 differentiation (stage 6, day 14). mCFP is an unstained control. Data represent mean ± SEM, ** p < 0.01 ( n = 3 biologically independent samples per group). e Proportion of Ki67 + in control or verteporfin-treated differentiating progenitors as assayed by flow cytometry at the end of stage 6 differentiation. Data represent mean ± SEM, *** p < 0.001 ( n = 3 biologically independent samples per group). f – h Flow cytometry analysis of SOX9 in non-targeting control and YAP shRNA-expressing pancreatic progenitors and quantification of SOX9 + cell proportion ( h ) at the end of stage 6 differentiation. Data represent mean ± SEM, *** p < 0.001 ( n = 3 biologically independent samples per group). i Experimental design for j – n . j , k in vivo GSIS of control and verteporfin-derived SC-β cell grafts as assayed by serum human insulin before and 30 min after a glucose injection 8 weeks post transplantation. l , m Immunofluorescence micrographs of grafts 12 weeks post transplantation of control and verteporfin-derived SC-β cell grafts stained for C-peptide and SOX9. Representative images and cropped blots (bottom panels) are shown. Asterisk (*) denotes nonspecific staining. Scale bar: 50 µm. n Quantification of SOX9 + of DAPI + cells within grafts 12 weeks after transplantation. Data represent mean (center line) ± min to max (whiskers) and lower and upper quartiles (bounds of box), *** p < 0.001, two-sided student’s t test ( n = 4 animals per group, values correspond to the average proportion of SOX9 + cells from four histological sections per animal)

Journal: Nature Communications

Article Title: YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing β cells

doi: 10.1038/s41467-019-09404-6

Figure Lengend Snippet: YAP inhibition reduces Sox9 + progenitors in vitro. a Experimental design for b – e . b – d Flow cytometry of SOX9 expression in the control and verteporfin-treated differentiating progenitors and quantification of the proportion of SOX9 + cells from b and c at the end of stage 6 differentiation (stage 6, day 14). mCFP is an unstained control. Data represent mean ± SEM, ** p < 0.01 ( n = 3 biologically independent samples per group). e Proportion of Ki67 + in control or verteporfin-treated differentiating progenitors as assayed by flow cytometry at the end of stage 6 differentiation. Data represent mean ± SEM, *** p < 0.001 ( n = 3 biologically independent samples per group). f – h Flow cytometry analysis of SOX9 in non-targeting control and YAP shRNA-expressing pancreatic progenitors and quantification of SOX9 + cell proportion ( h ) at the end of stage 6 differentiation. Data represent mean ± SEM, *** p < 0.001 ( n = 3 biologically independent samples per group). i Experimental design for j – n . j , k in vivo GSIS of control and verteporfin-derived SC-β cell grafts as assayed by serum human insulin before and 30 min after a glucose injection 8 weeks post transplantation. l , m Immunofluorescence micrographs of grafts 12 weeks post transplantation of control and verteporfin-derived SC-β cell grafts stained for C-peptide and SOX9. Representative images and cropped blots (bottom panels) are shown. Asterisk (*) denotes nonspecific staining. Scale bar: 50 µm. n Quantification of SOX9 + of DAPI + cells within grafts 12 weeks after transplantation. Data represent mean (center line) ± min to max (whiskers) and lower and upper quartiles (bounds of box), *** p < 0.001, two-sided student’s t test ( n = 4 animals per group, values correspond to the average proportion of SOX9 + cells from four histological sections per animal)

Article Snippet: The antibodies used in the study were the following: rabbit anti-YAP (1:100, Cell Signaling Technology; 14074 S), mouse anti-YAP (1:100, Abnova; 89106308), rabbit anti-SOX9 (1:100, Cell Marque; AC-0284RUO), rat anti-C-peptide (1:200, Developmental Studies Hybridoma Bank; GN-ID4), mouse anti-NKX6.1 (1:100, Developmental Studies Hybridoma Bank; F55A12-supernatant), rabbit anti-Ki67 (1:100, Abcam; ab16667), sheep anti-NGN3 (1:50, R&D systems; AF3444), goat anti-PDX1 (1:100, R&D systems; AF2419), rabbit anti-CHGA (1:200, Novus Biologicals; NB120–15160), mouse anti-CHGA (1:200, Santa Cruz; sc-393941), mouse anti-glucagon (1:200, Abcam; ab82270), mouse anti-somatostatin (1:200, Santa Cruz; sc-55565), rabbit anti-cleaved Caspase-3 (1:100, Cell Signaling; 9661) and mouse anti-PCNA (1:100, Millipore; NA03).

Techniques: Inhibition, In Vitro, Flow Cytometry, Expressing, shRNA, In Vivo, Derivative Assay, Injection, Transplantation Assay, Immunofluorescence, Staining

Antibodies

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Targeting the Gdnf Gene in peritubular myoid cells disrupts undifferentiated spermatogonial cell development

doi: 10.1073/pnas.1517994113

Figure Lengend Snippet: Antibodies

Article Snippet: Species Dilution Primary ACTA2 Sigma-Aldrich, A5228 (Clone 1A4) Mouse, monoclonal 1:200 ACTA2-FITC Sigma-Aldrich, F3777 (Clone 1A4) Mouse, monoclonal 1:200 dtTomato Clontech, 632496 Rabbit, polyclonal 1:500 GATA4 Santa Cruz, SC-1237 Goat, polyclonal 1:300 GDNF ( d -20) Santa Cruz, SC-328 Rabbit, polyclonal 1:200 GFP Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 Rabbit, polyclonal 1:200 HSPA2 Eddy laboratory, Allen et al. PMID 3352605 Rabbit, polyclonal 1:1,000 KI67 Abcam, ab15580 Rabbit, polyclonal 1:200 KIT Abcam, ab65525 (Clone 2B8) Rat, monoclonal 1:100 SOX9 Cosmo Bio Co. Japan, KAL-KO608 Rabbit, polyclonal 1:200 ZBTB16 (PLZF, H-300) Santa Cruz, SC-22839 Rabbit, polyclonal 1:200 Secondary Alexa Fluor 488, Donkey anti-mouse IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A21202","term_id":"641355","term_text":"A21202"}} A21202 Donkey, polyclonal 1:200 Alexa Fluor 546, Donkey anti-rabbit IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A10040","term_id":"489103"}} A10040 Donkey, polyclonal 1:200 Alexa Fluro 647, Donkey anti-goat IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11056","term_id":"489255","term_text":"A11056"}} A11056 Donkey, polyclonal 1:200 DAB staining VECTSTAIN Elite ABC Kit (Rabbit IgG) Vector Laboratories, PK-6106 ImmPACT DAB Peroxidase Substrate Vector Laboratories, SK-4105 Vybrant apoptosis Assay Kit 6 (V23200) Annexin V + Alexa Fluor 350 Life Technology PI Life Technology Open in a separate window Antibodies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. S2. caption a7 ZBTB16 immunostaining of undifferentiated spermatogonia in testes from WT, Het, and cKO mice at 1, 2, 4, 8, and 12 wk. ( A ) No obvious differences are seen at 1 wk. ( B ) Fewer ZBTB16-positive cells were present in cKO testes compared to Het and WT at 2 wk. ( C – E ) ZBTB16-positive cells were rarely observed at 4 ( C ), 8 ( D ), and 12 ( E ) wk in cKO testes.

Techniques: Staining, Plasmid Preparation, Apoptosis Assay

The Gdnf mRNA and GDNF and FGF2 protein levels in testes of 8-wk-old mice. (A) The Gdnf mRNA levels in WT, Het, and cKO mice relative to Sox9 levels. (B) The GDNF protein levels in WT, Het, and cKO mice relative to INHBB levels. (C) The FGF2 protein levels in WT, Het, and cKO mice relative to INHBB levels. ANOVA analysis with Tukey test. *P < 0.05, **P < 0.01.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Targeting the Gdnf Gene in peritubular myoid cells disrupts undifferentiated spermatogonial cell development

doi: 10.1073/pnas.1517994113

Figure Lengend Snippet: The Gdnf mRNA and GDNF and FGF2 protein levels in testes of 8-wk-old mice. (A) The Gdnf mRNA levels in WT, Het, and cKO mice relative to Sox9 levels. (B) The GDNF protein levels in WT, Het, and cKO mice relative to INHBB levels. (C) The FGF2 protein levels in WT, Het, and cKO mice relative to INHBB levels. ANOVA analysis with Tukey test. *P < 0.05, **P < 0.01.

Article Snippet: Species Dilution Primary ACTA2 Sigma-Aldrich, A5228 (Clone 1A4) Mouse, monoclonal 1:200 ACTA2-FITC Sigma-Aldrich, F3777 (Clone 1A4) Mouse, monoclonal 1:200 dtTomato Clontech, 632496 Rabbit, polyclonal 1:500 GATA4 Santa Cruz, SC-1237 Goat, polyclonal 1:300 GDNF ( d -20) Santa Cruz, SC-328 Rabbit, polyclonal 1:200 GFP Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 Rabbit, polyclonal 1:200 HSPA2 Eddy laboratory, Allen et al. PMID 3352605 Rabbit, polyclonal 1:1,000 KI67 Abcam, ab15580 Rabbit, polyclonal 1:200 KIT Abcam, ab65525 (Clone 2B8) Rat, monoclonal 1:100 SOX9 Cosmo Bio Co. Japan, KAL-KO608 Rabbit, polyclonal 1:200 ZBTB16 (PLZF, H-300) Santa Cruz, SC-22839 Rabbit, polyclonal 1:200 Secondary Alexa Fluor 488, Donkey anti-mouse IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A21202","term_id":"641355","term_text":"A21202"}} A21202 Donkey, polyclonal 1:200 Alexa Fluor 546, Donkey anti-rabbit IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A10040","term_id":"489103"}} A10040 Donkey, polyclonal 1:200 Alexa Fluro 647, Donkey anti-goat IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11056","term_id":"489255","term_text":"A11056"}} A11056 Donkey, polyclonal 1:200 DAB staining VECTSTAIN Elite ABC Kit (Rabbit IgG) Vector Laboratories, PK-6106 ImmPACT DAB Peroxidase Substrate Vector Laboratories, SK-4105 Vybrant apoptosis Assay Kit 6 (V23200) Annexin V + Alexa Fluor 350 Life Technology PI Life Technology Open in a separate window Antibodies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. S2. caption a7 ZBTB16 immunostaining of undifferentiated spermatogonia in testes from WT, Het, and cKO mice at 1, 2, 4, 8, and 12 wk. ( A ) No obvious differences are seen at 1 wk. ( B ) Fewer ZBTB16-positive cells were present in cKO testes compared to Het and WT at 2 wk. ( C – E ) ZBTB16-positive cells were rarely observed at 4 ( C ), 8 ( D ), and 12 ( E ) wk in cKO testes.

Techniques:

Sertoli cell numbers, seminal vesicle weights, and the GDNF expression of 8-wk-old WT, Het, and cKO mice. (A) Sertoli cell nuclei (arrows) were immunostained with rabbit anti-mouse SOX9 antibody. (B) The numbers of Sertoli cells per tubule cross-sections of WT, Het, and cKO were not significantly different. (C) The seminal vesicle weights of WT, Het, and cKO mice were not significantly different. (D) The expression of GDNF in Sertoli cells of WT, Het, and cKO mice. Sections were immunostained with rabbit antiserum to GDNF and Alexa Fluor 546 donkey anti-rabbit antiserum (red) and with FITC-conjugated mouse monoclonal antibody to ACTA2 (green) to determine which cell types contained GDNF. GDNF was localized to Sertoli cells and PM cells in WT and Het mice but only in Sertoli cells in cKO mice. Nuclei were stained with DAPI. (Scale bar: 50 µm.)

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Targeting the Gdnf Gene in peritubular myoid cells disrupts undifferentiated spermatogonial cell development

doi: 10.1073/pnas.1517994113

Figure Lengend Snippet: Sertoli cell numbers, seminal vesicle weights, and the GDNF expression of 8-wk-old WT, Het, and cKO mice. (A) Sertoli cell nuclei (arrows) were immunostained with rabbit anti-mouse SOX9 antibody. (B) The numbers of Sertoli cells per tubule cross-sections of WT, Het, and cKO were not significantly different. (C) The seminal vesicle weights of WT, Het, and cKO mice were not significantly different. (D) The expression of GDNF in Sertoli cells of WT, Het, and cKO mice. Sections were immunostained with rabbit antiserum to GDNF and Alexa Fluor 546 donkey anti-rabbit antiserum (red) and with FITC-conjugated mouse monoclonal antibody to ACTA2 (green) to determine which cell types contained GDNF. GDNF was localized to Sertoli cells and PM cells in WT and Het mice but only in Sertoli cells in cKO mice. Nuclei were stained with DAPI. (Scale bar: 50 µm.)

Article Snippet: Species Dilution Primary ACTA2 Sigma-Aldrich, A5228 (Clone 1A4) Mouse, monoclonal 1:200 ACTA2-FITC Sigma-Aldrich, F3777 (Clone 1A4) Mouse, monoclonal 1:200 dtTomato Clontech, 632496 Rabbit, polyclonal 1:500 GATA4 Santa Cruz, SC-1237 Goat, polyclonal 1:300 GDNF ( d -20) Santa Cruz, SC-328 Rabbit, polyclonal 1:200 GFP Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 Rabbit, polyclonal 1:200 HSPA2 Eddy laboratory, Allen et al. PMID 3352605 Rabbit, polyclonal 1:1,000 KI67 Abcam, ab15580 Rabbit, polyclonal 1:200 KIT Abcam, ab65525 (Clone 2B8) Rat, monoclonal 1:100 SOX9 Cosmo Bio Co. Japan, KAL-KO608 Rabbit, polyclonal 1:200 ZBTB16 (PLZF, H-300) Santa Cruz, SC-22839 Rabbit, polyclonal 1:200 Secondary Alexa Fluor 488, Donkey anti-mouse IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A21202","term_id":"641355","term_text":"A21202"}} A21202 Donkey, polyclonal 1:200 Alexa Fluor 546, Donkey anti-rabbit IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A10040","term_id":"489103"}} A10040 Donkey, polyclonal 1:200 Alexa Fluro 647, Donkey anti-goat IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11056","term_id":"489255","term_text":"A11056"}} A11056 Donkey, polyclonal 1:200 DAB staining VECTSTAIN Elite ABC Kit (Rabbit IgG) Vector Laboratories, PK-6106 ImmPACT DAB Peroxidase Substrate Vector Laboratories, SK-4105 Vybrant apoptosis Assay Kit 6 (V23200) Annexin V + Alexa Fluor 350 Life Technology PI Life Technology Open in a separate window Antibodies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. S2. caption a7 ZBTB16 immunostaining of undifferentiated spermatogonia in testes from WT, Het, and cKO mice at 1, 2, 4, 8, and 12 wk. ( A ) No obvious differences are seen at 1 wk. ( B ) Fewer ZBTB16-positive cells were present in cKO testes compared to Het and WT at 2 wk. ( C – E ) ZBTB16-positive cells were rarely observed at 4 ( C ), 8 ( D ), and 12 ( E ) wk in cKO testes.

Techniques: Expressing, Staining

PCR primers

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Targeting the Gdnf Gene in peritubular myoid cells disrupts undifferentiated spermatogonial cell development

doi: 10.1073/pnas.1517994113

Figure Lengend Snippet: PCR primers

Article Snippet: Species Dilution Primary ACTA2 Sigma-Aldrich, A5228 (Clone 1A4) Mouse, monoclonal 1:200 ACTA2-FITC Sigma-Aldrich, F3777 (Clone 1A4) Mouse, monoclonal 1:200 dtTomato Clontech, 632496 Rabbit, polyclonal 1:500 GATA4 Santa Cruz, SC-1237 Goat, polyclonal 1:300 GDNF ( d -20) Santa Cruz, SC-328 Rabbit, polyclonal 1:200 GFP Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 Rabbit, polyclonal 1:200 HSPA2 Eddy laboratory, Allen et al. PMID 3352605 Rabbit, polyclonal 1:1,000 KI67 Abcam, ab15580 Rabbit, polyclonal 1:200 KIT Abcam, ab65525 (Clone 2B8) Rat, monoclonal 1:100 SOX9 Cosmo Bio Co. Japan, KAL-KO608 Rabbit, polyclonal 1:200 ZBTB16 (PLZF, H-300) Santa Cruz, SC-22839 Rabbit, polyclonal 1:200 Secondary Alexa Fluor 488, Donkey anti-mouse IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A21202","term_id":"641355","term_text":"A21202"}} A21202 Donkey, polyclonal 1:200 Alexa Fluor 546, Donkey anti-rabbit IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A10040","term_id":"489103"}} A10040 Donkey, polyclonal 1:200 Alexa Fluro 647, Donkey anti-goat IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11056","term_id":"489255","term_text":"A11056"}} A11056 Donkey, polyclonal 1:200 DAB staining VECTSTAIN Elite ABC Kit (Rabbit IgG) Vector Laboratories, PK-6106 ImmPACT DAB Peroxidase Substrate Vector Laboratories, SK-4105 Vybrant apoptosis Assay Kit 6 (V23200) Annexin V + Alexa Fluor 350 Life Technology PI Life Technology Open in a separate window Antibodies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. S2. caption a7 ZBTB16 immunostaining of undifferentiated spermatogonia in testes from WT, Het, and cKO mice at 1, 2, 4, 8, and 12 wk. ( A ) No obvious differences are seen at 1 wk. ( B ) Fewer ZBTB16-positive cells were present in cKO testes compared to Het and WT at 2 wk. ( C – E ) ZBTB16-positive cells were rarely observed at 4 ( C ), 8 ( D ), and 12 ( E ) wk in cKO testes.

Techniques:

The mRNA levels for markers of undifferentiated spermatogonia in testes of WT, Het, and cKO 1-wk-old mice relative to Sox9 levels. (A) Bcl6b. (B) Zbtb16. (C) Nanos2. ANOVA analysis with Tukey test. *P < 0.05, **P < 0.01.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Targeting the Gdnf Gene in peritubular myoid cells disrupts undifferentiated spermatogonial cell development

doi: 10.1073/pnas.1517994113

Figure Lengend Snippet: The mRNA levels for markers of undifferentiated spermatogonia in testes of WT, Het, and cKO 1-wk-old mice relative to Sox9 levels. (A) Bcl6b. (B) Zbtb16. (C) Nanos2. ANOVA analysis with Tukey test. *P < 0.05, **P < 0.01.

Article Snippet: Species Dilution Primary ACTA2 Sigma-Aldrich, A5228 (Clone 1A4) Mouse, monoclonal 1:200 ACTA2-FITC Sigma-Aldrich, F3777 (Clone 1A4) Mouse, monoclonal 1:200 dtTomato Clontech, 632496 Rabbit, polyclonal 1:500 GATA4 Santa Cruz, SC-1237 Goat, polyclonal 1:300 GDNF ( d -20) Santa Cruz, SC-328 Rabbit, polyclonal 1:200 GFP Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 Rabbit, polyclonal 1:200 HSPA2 Eddy laboratory, Allen et al. PMID 3352605 Rabbit, polyclonal 1:1,000 KI67 Abcam, ab15580 Rabbit, polyclonal 1:200 KIT Abcam, ab65525 (Clone 2B8) Rat, monoclonal 1:100 SOX9 Cosmo Bio Co. Japan, KAL-KO608 Rabbit, polyclonal 1:200 ZBTB16 (PLZF, H-300) Santa Cruz, SC-22839 Rabbit, polyclonal 1:200 Secondary Alexa Fluor 488, Donkey anti-mouse IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A21202","term_id":"641355","term_text":"A21202"}} A21202 Donkey, polyclonal 1:200 Alexa Fluor 546, Donkey anti-rabbit IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A10040","term_id":"489103"}} A10040 Donkey, polyclonal 1:200 Alexa Fluro 647, Donkey anti-goat IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11056","term_id":"489255","term_text":"A11056"}} A11056 Donkey, polyclonal 1:200 DAB staining VECTSTAIN Elite ABC Kit (Rabbit IgG) Vector Laboratories, PK-6106 ImmPACT DAB Peroxidase Substrate Vector Laboratories, SK-4105 Vybrant apoptosis Assay Kit 6 (V23200) Annexin V + Alexa Fluor 350 Life Technology PI Life Technology Open in a separate window Antibodies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. S2. caption a7 ZBTB16 immunostaining of undifferentiated spermatogonia in testes from WT, Het, and cKO mice at 1, 2, 4, 8, and 12 wk. ( A ) No obvious differences are seen at 1 wk. ( B ) Fewer ZBTB16-positive cells were present in cKO testes compared to Het and WT at 2 wk. ( C – E ) ZBTB16-positive cells were rarely observed at 4 ( C ), 8 ( D ), and 12 ( E ) wk in cKO testes.

Techniques:

The mRNA levels for markers of differentiated spermatogonia in testes of WT, Het, and cKO 1-wk-old mice relative to Sox9 levels. (A) Ngn3. (B) Kit. (C) Nanos3. (D) Spo11. (E) Dnmt3l. ANOVA analysis with Tukey test. *P < 0.05.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Targeting the Gdnf Gene in peritubular myoid cells disrupts undifferentiated spermatogonial cell development

doi: 10.1073/pnas.1517994113

Figure Lengend Snippet: The mRNA levels for markers of differentiated spermatogonia in testes of WT, Het, and cKO 1-wk-old mice relative to Sox9 levels. (A) Ngn3. (B) Kit. (C) Nanos3. (D) Spo11. (E) Dnmt3l. ANOVA analysis with Tukey test. *P < 0.05.

Article Snippet: Species Dilution Primary ACTA2 Sigma-Aldrich, A5228 (Clone 1A4) Mouse, monoclonal 1:200 ACTA2-FITC Sigma-Aldrich, F3777 (Clone 1A4) Mouse, monoclonal 1:200 dtTomato Clontech, 632496 Rabbit, polyclonal 1:500 GATA4 Santa Cruz, SC-1237 Goat, polyclonal 1:300 GDNF ( d -20) Santa Cruz, SC-328 Rabbit, polyclonal 1:200 GFP Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 Rabbit, polyclonal 1:200 HSPA2 Eddy laboratory, Allen et al. PMID 3352605 Rabbit, polyclonal 1:1,000 KI67 Abcam, ab15580 Rabbit, polyclonal 1:200 KIT Abcam, ab65525 (Clone 2B8) Rat, monoclonal 1:100 SOX9 Cosmo Bio Co. Japan, KAL-KO608 Rabbit, polyclonal 1:200 ZBTB16 (PLZF, H-300) Santa Cruz, SC-22839 Rabbit, polyclonal 1:200 Secondary Alexa Fluor 488, Donkey anti-mouse IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A21202","term_id":"641355","term_text":"A21202"}} A21202 Donkey, polyclonal 1:200 Alexa Fluor 546, Donkey anti-rabbit IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A10040","term_id":"489103"}} A10040 Donkey, polyclonal 1:200 Alexa Fluro 647, Donkey anti-goat IgG Life Technologies, {"type":"entrez-nucleotide","attrs":{"text":"A11056","term_id":"489255","term_text":"A11056"}} A11056 Donkey, polyclonal 1:200 DAB staining VECTSTAIN Elite ABC Kit (Rabbit IgG) Vector Laboratories, PK-6106 ImmPACT DAB Peroxidase Substrate Vector Laboratories, SK-4105 Vybrant apoptosis Assay Kit 6 (V23200) Annexin V + Alexa Fluor 350 Life Technology PI Life Technology Open in a separate window Antibodies fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. S2. caption a7 ZBTB16 immunostaining of undifferentiated spermatogonia in testes from WT, Het, and cKO mice at 1, 2, 4, 8, and 12 wk. ( A ) No obvious differences are seen at 1 wk. ( B ) Fewer ZBTB16-positive cells were present in cKO testes compared to Het and WT at 2 wk. ( C – E ) ZBTB16-positive cells were rarely observed at 4 ( C ), 8 ( D ), and 12 ( E ) wk in cKO testes.

Techniques: